next content (in%):
Corn flour5.0Yeast autolysate0.5Water94.5 the dilute solution of sulphuric acid рН of medium is reached to 4,8. The prepared medium is poured out in shake-flask propagators on 500 ml per 300 ml in each and sterilize at a temperature 121-125 В° C during 40-60 min. After sterilization medium is cooled to 26 В° C and is inoculated with the suspension of conidia received at growing of culture on the slant agar medium in tests tubes. Flasks stand on shake-and-flask propagator. Growing is conducted at a temperature 24-26 В° C during36-48 h at frequency of vibrations of shake and-flask propagator 200-220 min - 1 .36-48 h (due to the conclusion of microbiologist) a liquid culture is passed on the second stage. Incubated liquid inoculum of the second stage is the inoculums for incubation of sowing culture. The volume of inoculums should be 0.5 - 1.0% to volume of nutrient medium in inoculator.
Inoculation of medium in fermenter
Before sowing from fermenter take the tests of medium through the sampler for the microbiological inoculation and biochemical analysis.sowing close a valve on an output air-track at a sowing vehicle and lift pressure to 0,06 - 0,08 МPа, and in fermenter remains pressure 0,02-0,03 МPа. After this open a valve on the line of pressing at a sowing vehicle and fermenter and due to the difference of pressure press inoculum into a fermenter. br/>В
a valve on the line of pressing, turn on a mixer and begin the process of culture growing in fermenter. After termination of cultivation the line of pressing is steamed thoroughly. The amount of inoculum is 3% to the volume of nutrient medium in fermenter. [1] of Asp. awamori culture
Process of growing of submerged culture of Asp.awamori in a production is carried out in fermenters from stainless steel in sterile conditions at continuous agitation and aeration of medium. Fermenter in which grows the submerged culture is provided with a shirt for heating and cooling, with an aerating device, jets for steam supply, inoculating and drain lines, bushing for a manometer and thermometer, sampling knot, anti-foaming tank and individual air filter . [20] microbiological and biochemical control of development of culture with the observance of all terms of sterility tests are taken from fermenter after 72 h after inoculation, and then every twenty-four hours of growth. In tests glucoamylase activity, рН, concentration of dry substances, state of culture and absence of extraneous microflora at microscopy is determined. [21]
process of fermentation proceed at next conditions:
Temperature of medium in fermenter,
