ify"> o C35Method of agitation double-level turbine stirrerFrequency of mixer rotations, min -1 150-170Quantity of air supplied, m 3 < span align = "justify">/m 3 of medium/hourno less than 30-60Pressure under the lid of fermenter during the growth, MPa0.02-0.03Temperature of air at inlet to fermenter, o C35-40 prepared culture must satisfy the next requirements:
Glucoamylase activity units/mlno less than 200Concentration of dry substances in the filtrate of culture,% 8-10pH3.0-3.5presence of extraneous microfloraabsent
.2.3 Micribiological and chemical control
Regardless of method of cultivation from the moment of inoculation with producer a sterile nutrient medium the control for growth of culture and production of enzymes is conducted. For every species of producer and method of cultivation, the periodicity of selection of middle tests of growing culture is set. The selected tests are exposed to microscopy and visual examination. With the purpose of exposure of possible infections the periodic inoculation of tests on agar media with introduction of factors, repressing the growth of producer is made. Determination of accumulation in the culture of fermentative activity is constantly conducted. At submerged cultivation control for the consumption of basic limiting substrates of medium (carbohydrates, N, Р) is conducted, рН of culture is measured.indexes of growth of culture, the changes of composition of medium and accumulation of enzymes, etc. are added to the laboratory magazine.all stages of selection of enzymes conduct the analyses of activity, the sizes of losses and output of commodity product is determined. The prepared preparations of enzymes expose to especially careful research, especially those which are used in medicine and in foods. Spores or cells of producer must be absent in the prepared product, and a maximum norm of semination by microflora is determined in every case.liquid and dry enzymatic preparations are analysed on activity of enzymes, contents of dry substances, presence of microbial contamination. At control of the high-cleared preparations besides determination of contamination with microbes and activity of enzymes analyses on content of protein, ash elements, carbohydrates and other specific properties of enzymes is conducted. [1]
2.2.4 Processing of waste water and air
At submerged cultivation of glucoamylse producer Asp. awamori the waste products are air and water, which contain spores of the cultivated microorganism. At submerged cultivation of microorganisms in fermenters amount...
