cools down directly in fermenter. [18]
After filling of fermenter all system is released from medium, pump with a water for removing of suspended particles of nutrient medium and sterilize with sharp steam during 30-40 min at pressure 0,20-0,25 МPа. Liberation and puming of the system is carried out in an agitation tank. Water discharge must make 2-3 volumes of the system. br/>
.2.2 Technological processes
Preparation of inoculum
For inoculation of production nutrient medium at submerged cultivation inoculum is also prepared by a submerged method.receipt of inoculum is carried out by the phasic increase of mass culture of producer. At small productivity of workshops it is taken to one or two operations, and for the plants of the large productivity there is a sequential process.Аsp. awamori there are such stages of inoculums preparation:
. Test tube with an initial culture on agar nutrient medium.
. Subculturing of water suspension of culture in retorts with a liquid nutrient medium containing 5% of corn-flour and 0,5% of the autolyzed yeast, cultivation on shake -flask propagator during 48 h.
. Subculturing of culture in vessels with the capacity 6 l (quantity of inoculum 10-12% to the volume of nutrient medium), cultivation during 48 h.
. Subculturing of culture in an inoculator on corn wort with concentration 6%, cultivation during 48 h. at a temperature 27 В° C, interfusion with mixer with frequency of rotation 950 rotations/minute and with the air supply 16 m 3 /(m 3 /h)
. Subculturing of culture into productive fermenter (3% of inoculums to the volume of nutrient medium)
The inoculums material of Asp. awamori mold is prepared in laboratory in test tubes on agar nutrient medium with the next content (in%):
Glucose 2.0Sodium nitrate0.91Potassium chloride0, 05Magnesium sulphate 0.05Dihydroorthophosphate0.10Iron sulphate 0.001Distilled water 96.84 is sterilized during 40 minutes at pressure 0,1 МPа.culture of mold is inoculated on slant medium in test tubes and grow during 12 days in a thermostat at 25 В° С. The prepared culture must have a characteristic for species color and folded surface. It is used for preparation of liquid inoculating material. [19]
Preparation of liquid inoculume . liquid inoculum prepares in a few stages. By a three-phasic scheme on the first stage prepare the nutrient medium of the ...
