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Реферат Project of glucoamylase production by submerged cultivation of Aspergillus awamori





no less than 25%, and an ammonia of II brand is no less than 20% nitrogen. [17]

Preparation of nutrient medium

Nutrient medium is prepared, using corn mash with the concentration of dry substances 18-20%, which is received due to the next scheme: corn-flour through a portion automatic scales are loaded in a mixer, in which simultaneously and during permanent work of mixer water with a temperature no more than 45 В° С is supplied. Ratio of flour and water 1: (2,5-3,0). <В 

mass is pumped with a pump in a boiling apparatus, working under the pressure, mass in a vehicle is heated by sharp steam, which is entered through the bottom. Boiling soft is produced at temperature148-154 В° C and pressure 0,36-0,44 МPа 15-20 min. [12] boil soft a flour is possible both in the vehicles of batch-type and by continuous method in the boiling apparatus of tube-type, to which steam is supplied by pressure no less than 0,5 МPа.

The boiled soft mass enters saccharifier, equipped with a worm-pipe for cooling. Before blowing of mass in a saccharifier water in an amount 5% to the volume of mass is added.boiled soft mass is cooled to 63 В° C, then saccharify with malt milk, which is received by mixing in the malt vats of the malt ground up on a maltcrusher and water. Correlation of malt and water 1: (6-8). Duration of saccharification at a temperature 58-60 o C during 30 min.

Got wort with the concentration of dry substances 18-20% and рН 5,3-5,6 by a pump is feeded on a contact head, pumped through it, warmed up by sharp steam to 85 В° C and given in a sterilizer 15.

В 


of nutrient medium is carried out on setting, including a contact head, tubular holder and heat-exchanger.Before sterilization of medium the system is checked for impermeability by steam under the pressure 0,20 - 0, 25 МPа. At finding out leaking of steam pressure is reduced to zero, defects are removed and the system is again checked up on hermeticity. During the complete pressurizing the system is sterilized by sharp steam during 30-40 min at pressure 0,2-0,25 МPа. After termination of sterilization of the systems proceed the sterilization of medium. Nutrient medium with temperature 75-80 В° C by a piston pump given through a contact head, where heated to the temperature 120-125 В° C, in a tubular holder-sterilizer, where 30-40 minutes is maintained, then cools down in heat -exchanger to 35 В° C and enters the fermenter.work of heat-exchanger the by-pass line, valves of water and medium supply must be under steam defence. In absence of heat-exchanger a nutrient medium directly from holder enters fermenter. In the process of its filling pressure 0,10-0,12 МPа is supported in a vehicle. Sharp steam at this time is giventhrough the system of aeration. Medium...


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