uid on preparation of solutions of nutrient salts and limewater. As a result the amount of exhaust cultural liquid decrease twice. [22]
.3 Description of equipment scheme. Specification of equipment
equipment scheme of the production of glucoamylase by submerged cultivation of Asp. awamori is shown on figure 6.saccharified corn mash enters collecting tank 1 from where by a centrifugal pump 2 it is pumped to the agitation tank 8 for preparation of nutrient medium. Other components for medium such as water and salts such as potassium phosphate and ammonium sulphate is supplied from collecting tank 3 are added in agitation tank 8.agitation tank components of nutrient medium is carefully mixed and рН of solution bring to 4.8 with sodium hydroxide. A medium is sterilized then, for what by a pump 9 it is given in a contact head 10, heat from 75-80 В° C to 125 В° C, maintain in a pipe-type holder 11 during 30-40 mines and cool to 30 -32 В° C in surface heat-exchanger 12.sterilized and cooled medium enters fermenter 13 that is a vertical cylindrical vessel with radial aerators or with two-level turbine stirrer and bubbler for air supply.the process of filling of fermenter an excess pressure 0 , 25 MPа is supported in it by a steam, supplied through the air duct through an aerating device. Fillfactor of fermenter is 0,75-0,85. At its less value a volume is taken to the norm by the supply of medium from agitation tank 8 through the system of sterilization. After filling of fermenter all system is released from a medium, water is pumped and sterilize with sharp steam. A nutrient medium in fermenter is cooled to 33-35 В° С.fermenter medium is inoculated by the culture of molds from manifold 14. Before inoculation from fermenter take samples through the sampler for microbiological control and biochemical analyses. Inoculation is carried out through a pressing line preliminary sterilized from manifold to fermenter by sharp steam during 1 hour. For this purpose valve on an output airline of manifold close and lift in it pressure to 0,06-0,08 MPa, leaving in fermenterе pressure 0,02-0,03 MPa, whereupon open a valve on the pressing line in manifold and fermenter and in result of pressure differences inoculum from manifold is pressed into a fermenter. After this close valves on the pressing line, in fermenter drive to the rotation a stirrer and begin the process of growing of culture.pressing of all inoculum culture from manifold let the air out, open a lid and carefully wash internal surface. Then manifold is sterilized and fill with a nutrient medium for the next cycle of preparation of inoculum.medium for manifold is prepared in an agitation tank 5, equipped with a stirrer. In the beginning in agitation tank add water, then turn stirrer and gradually supply salts and corn wort and vegetable oil if necessary. Stirring of medium is made not only by a stirrer, but also as a result ...
