of its circulation by pump 6.the same pump medium is given through a contact head 7 into manifold, where it is maintained during 1,5-2 h at 125 про С, cool to 33-35 В° C and inoculate with Asp. awamori spores, through sowing actuator acces with the maintenance of sterility and at minimum motion of air in a workshop. After inoculation open valves for inlet and outlet of air. Expense of air 30-60 m 3 /(m 3 -h), its temperature 35-40 В° С. Duration of cultivation 36 h.of air, supplied in manifold and fermenters, conduct as follows. Before pumping to the rotary liquid-packed ring compressor 17 air is purified from mechanical admixtures on a viscin filter 16, and after a compressor is released from moisture consequently in dehydrator 18 and moisture separator 19. The compressed and dried air is heated in heat-exchanger 20 to the temperature 60-80 В° C and then purify from a microflora on a general head filter 22, filled with a basaltic fibre. After a head filter air is additionally purified on individual filters 22 at manifold and 23 at fermenter, which are also filled with a basaltic fibre.filters sterilize simultaneously with manifolds and fermenters by sharp steam during 2 h at excess pressure about 0.2 - 0.3 MPa. Moisture is removed from filters by blowing air through them.cultivation of molds in fermenter temperature of nutrient medium 35 В° C is supported by automatic control of water supply in the shirt of apparatus. Aeration and agitation with stirrer (frequency of rotation about 150 - 170 rotations/minute) is conducted continuously from the moment of inoculation completion and to the end of fermentation. Amount of the air supplied is 30-60 m 3 /(m 3 -h). Sampler and lower draining communication are under steam defence. Duration of the fermentation is 120-160 h.
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air from fermenter and manifold through the nozzle in a lid is thrown out through scrubber 24 in an atmosphere. In scrubber air is purified from spores and other suspended particles.liquid by a pump 15 is pumped for further processing and obtaining of enzyme preparation. Empty fermenter is washed and sterilized at 120 В° C during 2 hours.the process of fermentation for microbiological and biochemical control of development of culture take samples (with the maintenance of sterility) in 24 hours after inoculation and then every 12 hours of growth. The prepared culture must have activity of glucoamylase no less than 200 units/ml. [20] of the equipment is given in the table 5. br/>
Table 5. Specification of equipment of technological scheme for glucoamylase production
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