hat is widely used in food and agricultural industry, that is for beverages and feed additives production. The most feasible and efficient method of this enzyme production is microbial synthesis. According to reviewed literature the best microorganism for glucoamylase production is mold Asp. awamori because of its high activity for biosynthesis.
. The best method for the production of glucoamylase by cultivation of Asp.awamori is submerged fermentation on the corn mash substrate, because of easier controlling of parameters, minimal requirements of hand labor, low cost of raw material and possibility of sufficient providing medium with Oxygen due to requirements of aerobic culture.
. Technological process of glucoamylase production in department of biosynthesis includes the several stages, such as additional works (preparation of equipment, water, air sterilization, disinfectants and nutrient medium preparation), preparation of inoculums and actually fermentation. And according to these stages technological and equipment schemes of glucoamylase production by submerged cultivation were developed. p>. Time of medium holding in heating head for sterilization 27 m3 of nutrient medium from 30 to 130oC with volumetric discharge of steam 710 m3/h is 1.36 sec and quantity of orifices with diameter 2 mm for inlet of steam into medium is 294 .
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