Quantification of osteocyte lacunar density and anisotropy of vascular canals in compact bone
В
-Introduction, bone structure, remodelation, osteoporosis,
Remodelling of Haversian bone may result in both spatial and temporal variability of osteocyte lacunar density and arrangement of vascular canals. p> -Parameters and methods in bone histomorphometry, tetracycline labeling, disector
The most commonly used histomorphometric parameters: [1]
1 - Structural parameters
Measures that describe the biopsy specimen
-Core width (mm)
C.Wi Overall size of the biopsy specimen, refers to the width of the entire biopsy specimen or core. It is the distance between the inner and outer periosteal surfaces
-Cortical width (Оњm)
Ct.Wi Distance between periosteal and endocortical surfaces, he sum of the widths of inner and outer cortices of the biopsy specimen.
-Bone volume/tissue volume (%)
BV/TV Space taken up by mineralized and unmineralized bone relative to the total size of a bone compartmen, is cancellous bone volume as a percentage of tissue volume. The numerator includes matrix, whether mineralized or not, and the denominator includes cancellous bone, marrow, and associated soft tissue.
Measures that describe the configuration of trabeculae in space:
Trabecular thickness (Ојm)
Tb.Th Is the mean distance across individual trabeculae
Trabecular number (/ Mm)
Tb.N Number of trabeculae that a line through a trabecular compartment would hit per millimeter of its length, (BV/TV)/Tb.Th
- Trabecular separation
Tb.Sp Is the mean distance between individual trabeculae
2 - Static formation parameters
Osteoid thickness (Оњm)
O.Th Distance between the surface of the osteoid seam and mineralized bone
Osteoid surface/bone surface (%)
OS/BS Percentage of bone surface covered by osteoid
Osteoid volume/bone volume (%)
OV/BV Percentage of bone volume consisting of unmineralized osteoid
Osteoblast surface/bone surface (%)
Ob.S/BS Percentage of bone surface covered by osteoblasts
Wall thickness (Оњm)
W.Th Mean thickness of bone tissue that has been deposited at a remodeling site, is the mean distance between resting trabecular surfaces and cement lines
3 - Dynamic formation parameters
Mineralizing surface/bone surface (%)
MS/BS Percentage of bone surface showing mineralizing activity
Mineral apposition rate (Ојm/d)
MAR Distance between two tetracycline labels divided by the length of the labeling interval
Mineralization lag time (d)
Mlt Time interval between the deposition and mineralization of matrix
Bone formation rate/bone surface (Ојm3 Г— Ојm-2 * y-1)
<...
