erigmas are covered on all surface. Sterigmas are mainly double-level, metulas 6-16 х 3,5 -7 ? m. Conidium is spherical, 3,5-6 ? m.culture of strain assimilates glucose, saccharose, arabinose well, and poorly - maltose, lactose, lactoglucose and ramnose. Starch hydrolyze to glucose. Well assimilates ammoniacal salts of inorganic acids. It consumes a peptone, casein, amino acid, peptonizes milk.disadvantages of the described strain is a necessity for the receipt of high enough activity of cultural liquid, use of multiphase preparation of inoculum and enriched cultural medium for the basic fermentation process. [9 ] enzyme glucoamylase is commercially valuable biological product that is widely used in food and agricultural industry, that is for beverages and feed additives production. The most feasible and efficient method of this enzyme production is microbial synthesis. According to reviewed literature the best microorganism for glucoamylase production is mold Asp. awamori because of its high activity for biosynthesis.
general notion microorganism glucoamylase
2. Technological process
.1 Grounds for choosing technological scheme
biotechnological production of enzymes is realized by two methods - surface and submerged. The first method, applied for cultivation of molds is characterized by development of mycelium on a surface of solid or liquid substrate. The film of mycelium, producing not only amylolytic enzymes but also organic acids, inactivating them appears on liquid substrate, therefore solid substrates with the developed surface - wheat bran, pellet of grains, potato fiber and others are used. Maximal activity of enzymes is reached at cultivation of molds on wheat bran. The pellet of grains is poor in nutrient substances, and activity of enzymes in the cultures of molds, grown on it in 4-5 times lower, than on bran. The mature culture of molds in result of bran particles covering with mycelium looks like dense felt-like mass. [10] surface fermentation consists in growth of producer on the surface of thin layer of solid loose medium. Submerged fermentation in a liquid medium can be realized both in the conditions of batch process and with the use of the flowing systems.surface fermentation for the receipt of inoculum spore material is propageted by a superficial method or museum culture is grow in the conditions of submerged liquid culture. Further inoculum is sent to the stage of fermentation, which comes true on the surface of loose medium in metallic trays or vertical perforated cuvettes. A culture develops on the surface of solid loose medium, the basis of which is wheat bran, grain-growing husk, being the source of growth substances. For loosening of medium the arboreal sawdusts (5-10...
