rilized by sharp steam at a temperature 105-120 В° C and excess pressure 0,05-0, 1 МPа., especially inoculation boxing, is sterilized by irradiation by means of the special bactericidal lamps. Sterilization of apparatus and communications has significant mean at the submerged method of cultivation. The most careful sterilization can not give an effect, if impermeability of equipment is broken. [12] valves before setting check up by hydraulic compression at pressure 0,3 МPа. Impermeability of connections is checked up at excess pressure of steam 0,15 - 0,2 МPа. The special attention is made to sterilization of apparatus and communication for the serve of the defoamer. Sterilization of these knots is conducted at 125-135 В° C during 1,5-2 h. On the stage of sterilization permanent microbiological control of sterility of nutrient medium, air supplied to fermenter, defoamer etc is conducted.process of fermentation for defoaming in apparatus liquid defoamer is supplied. For receiving 0,05% emulsion of defoamer, in a capacity bring in its concentrate, then dilute it to necessary concentration. Emulsion of defoamer is sterilised in the special vehicle of batch-type at temperature 123 В± 2 В° C during 30 min in order to avoid bringing with it infections to medium. After sterilization defoamer is cooled in the same apparatus to temperature 30-32 В° C, then supply through a metering device in a fermenter and inoculator. [7] a microbiological sterility check up the department of sterilization, its walls and floor, apparatus, communications , and also hands of workers.
Preparation of air
The producer of glucoamylase enzyme Asp.awamori is an aerobe, and for its normal development in the process of cultivation it is necessary to give sterile air in a sufficient amount. Especially high demands to sterility is required at preparation of air for aeration of submerged culture.are a few methods of cleaning and sterilization of air, based on two principles: killing of microorganisms and their mechanical separation. Preparation of air for aeration is conducted as follows:
cleaning air from rough mechanical suspended particles (viscin filters)
preliminary conditioning to the necessary temperature
air supply in a compressor
thin cleaning of air from microorganisms (head filter)
final cleaning in individual filter.the stage of pre-cleaning of air the bulk of large dust particles with the diameter 5-10 ? m is removed. As filters of pre-cleaning use oily filters. For a compression and injection of air use turbo-compressors in which the compression of air takes place under the action of centrifugal force. The compression of air is accompanied by its heating to 220 o С....
