n of fermentation for prevention of inactivation of enzymes cultural liquid is cooled and is directed to down stream. p align="justify"> Basic difficulty in realization of continuous cultivation is a large danger of infecting, and necessity of frequent shutoff for realization prophylactic sterilization.
Table 3. Comparison of surface and submerged cultivations
SurfaceSubmergedRequires much space for trays Requires much hand labor Uses lower pressure air blower Little power requirement Minimum control necessary Little contamination problem Recovery involves extraction with aqueous solution, filtration or centrifugation, and perhaps evaporation and/or precipitationUses compact fermenters Requires minimum of labor Requires high pressure air Needs considerable power for air compressors and agitators Requires careful control Contamination frequently a serious problem Recovery involves filtration or centrifugation, and perhaps evaporation and/or precipitation submerged cultivation microorganisms develop in all volume of liquid nutrient medium. Because majority of producers of enzymes is obligate aerobs, a medium is intensively aerated. In microorganisms occures two indissolubly constrained processes that is a synthesis of biomass and synthesis of enzymes. [1]
For the maximal accumulation of enzymes certain composition of nutrient medium, providing of air with Oxygen, timely taking off of metabolites and physiological heat, optimal values ​​of рН and temperatures is needed. A major condition is also sterility of nutrient medium, supplied air, fermenters, pipelines and fittings., The best method for the production of glucoamylase by cultivation of Asp.awamori is submerged fermentation, because of easier controlling of parameters, minimal requirements of hand labor , low cost of raw material and possibility of sufficient providing medium with Oxygen due to requirements of aerobic culture.
.2 Description of the technological scheme
process of glucoamylase production in department of biosynthesis include the next main stages:
В· Additional works
В· Preparation of inoculums
В· Fermentation [Apendix C]
2.2.1 Additional works
Preparation of equipmentsurface cultivation it is necessary sterilize an apparatus for preparation of inoculum (capacities for inoculation, cuvettes, capacity for water, for preparation of inoculum suspension, inoculums communications). Sterilization of cuvettes and glassware in an inoculation department is conducted by dry steam at a temperature 160 В° C no less than 60 min Apparatus and communications are ste...
