Therefore after compressors air enters refrigerator. To delete excess moisture from air, it must be cooled.air enters head filter of КБ ВНІІФСа, that is a steel cylinder with a spherical bottom and sectional lid. Inside it the nets between which fiberglass filter material is fixed are located. A filter is sterilized by steam with pressure 0,2 МPа at 133 В° C during 3 hours. Reupholstering of head filter is made once per 2-3 months. [13]
В
cleared air enters individual filters for the thin cleaning and given for aeration of growing culture in an inoculator and fermenter. For a fermenter the filter of лаїків СП6/15 is used, for inoculums - filter ФТО - 60. Filter material, used for the filters of the thin cleaning, has a coefficient of skip 1 ? 10 9 %, that provides the required sterilization of air, necessary for development of microorganisms. Filters are sterilized with steam. [7]
Preparation of water
For preparation of medium water is taken from water supply system, artesian mining holes or open reservoirs after corresponding treatment. It must be biologically clean (ГОСТ 2874-82), colourless, without taste and smell, must not give precipitate. The dry residue of water must not exceed 1000 mg-eq/l, general hardness must not be more than 7 mg-eq/l. Too hard water slows the growth of microorganisms, because dissolved in it substances are not taken into account in composition of nutrient medium for a certain culture. [13] is necessary to check up chemical composition of water. Maintenance of gypsum is undesirable if there are more than 0,5 g/l and presence of salts of ammonium, because they testify that decaying processes take place.quantity of microorganisms in 1 ml of water must not be more than 100. In microbiological industry water is used not only for preparation of mediums but also for washing of apparatus, systems of cooling, etc. [14] must not exceed next concentrations:
4. Contents of harmful substances in water
SubstanceContent, g/lLead0.1Arsenic0.05Fluorine1.5Zink5.0Copper3.0
In the real time by a basic normative document, qualificatory quality of drinking-water, there is ГОСТ 2874-82 "Water drinkable. Hygienic requirements and control after quality ". From 01.01.2000 in Ukraine a new normative document is put in an operation the State sanitary rules and norms (ДСанПіН) № 383 (186/1940) "Water drinkable. Hygienic requirements to water quality of centralized household water use ". In a microbiological production presence of clean water in large quantity has a great value. [15]
Preparation of disinfectants
The basic requirement to the desinfectants is the effective removing of al...
