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Реферат Project of glucoamylase production by submerged cultivation of Aspergillus awamori





itionNotationNamesQuantityNote Documentation AD 04.000Assembly drawing1 304.001Collecting tank22 4 1504.002Centrifugal pump36 904.006Pump25 804.005Agitation tank27 1004.007Contact head21104. 011Pipe-type holder11204.012Surface heat-exchanger11304.013Fermenter11404.014Manifold11604.016Viscin filter11704.017Rotary liquid-packed ring compressor11804.018Dehydrator11904.019Moisture separator12004.020Heat-exchanger12104.021Head filter12204.022Individual filter at manifold12304.023Individual filter at manifold12404.024Scrubber1

3. DESCRIPTION AND CALCULATION OF HEATER


.1 Description of heater for nutrient medium supplied to fermenter

of the conditions of influence on biologically active substances biosynthesis is ensuring of production sterility and as well as nutrient medium components sterility. At biologically active substances production different multicomponent nutrient mediums in which foreign microorganisms may be present are used. These microorganisms should be fully disrupted or killed. Sterilization is the process of complete disruption or elimination of microorganisms.of sterilization is destruction of all microflora in the nutrient medium, different liquid admixtures. Necessity of sterilization is caused because cultures those are producers of enzymes or other biologically active substances very sensitive to other organisms presence.of molds for production of enzymes are cultivated on sterile nutrient mediums.process can be divided into three stages: medium or apparatus heating to the temperature of sterilization, holding this temperature during some time providing death of all microorganisms and the last - cooling of sterilized object to the temperatures available for inoculation of medium by pure culture of producent. Here is described apparatus for heating of medium to necessary temperature of sterilization. [18] the sterilization of medium it is heated to 130 В° С and is held at this temperature for 15 minutes. Heating proceeds in contact head that is built in the pipeline through which from agitation tank medium enters fermenter or manifold.cylindrical body of apparatus (diameter 159/149 mm) the pipe is built in (diameter 112 mm) with apertures with diameter 2mm, in screw facet, turn across external surface of glass with height 290 mm. In circular space between body and inner pipe through the connecting branch (diameter 57/70 mm) inject steam (P = 4kg/cm 2 ), which is after passing through the aperture penetrates product heating it to temperature of sterilization.sterilization of nutrient medium supplied to manifold sterilizers of similar construction is applied. [23]

.2. Calculation of heater of medium supplied to fermenteris heated in the column o...


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